As proposed by Drea et al. (2013), we performed a major elements analysis on the correlation matrix

As proposed by Drea et al. (2013), we performed a major elements analysis on the correlation matrix

Assortment of fragrance examples

After the Alpine marmots have been tranquilized, the anal glands had been washed with ethanol to eliminate any contaminants that are environmental. Then, we gathered odoriferous secretions by carefully squeezing the location across the gland, putting on clean nitrile gloves to prevent contamination by peoples chemical compounds. Each test had been gathered by having a 50 µL glass capillary and put in to a 1.5 mL opaque glass that is chromatographic filled up with 200 µL of dichloromethane pantyhose smokers solvent (HiPerSolv CHROMANORM for HPLC; VWR, Center Valley, PA, USA). In each 200 µL we included a standard that is internal biphenyl (molecular fat, 154.21 g mol −1 , 99.5%; Sigma Aldrich, St Louis, MO, United States Of America) at a concentration of 0.2 g L −1 . Vials had been sealed by having a cap that is teflon-lined kept at −20 °C into the industry and also at −80 °C into the laboratory until GC-MS analysis. As well as these fragrance samples, a few control that is‘field’ (in other words. with solvent but without marmot secretions) were collected with the same protocol, to spot possible contaminants.

Petrol chromatography–mass spectrometry analysis

Right before GC-MS analyses, we transferred all scent that is liquid (including ‘field control samples’) to 0.3 mL inserts in brand brand brand new, clean vials. Read more…